PALESTRA – Análise de Partículas e estudos de estabilidade por espalhamento de luz
Dra. Betty Petrillo
International Product Manager – Anton Paar GmbH
Date: March 24, 2017 – 09:00 – 12:00
Local: Auditório do IMA
Abstract: Particles – small, discrete portions of matter – surround us. Agrochemicals, injectable, drug delivery systems, advanced materials, consumer products that are used on a daily basis such as sun screen, cosmetics, shampoos/conditioners, kids’ toys etc., have nano- and micro-sized particles incorporated into them to improve their performance, strength, and stability.
Because such particles are now so commonly used, technologies for assessing their safety, environmental impact, stability etc. are critically important.
Dynamic light scattering (DLS) combined with Electrophoretic Light Scattering (ELS) and Static Light Scattering (SLS) have now become standard laboratory technologies for measuring the size, zeta potential, and molecular mass of particles ranging from the sub-nanometer to millimeter size range. More advanced DLS systems can automatically analyze the sample transmittance, or the amount of light traveling through the sample, enabling the user to assess certain information about the sample stability as well as to assess whether the sample is appropriate for DLS or ELS measurements.
One of the main questions when analyzing particles is about their stability, which represent the resistance of particles to aggregate over time. If your particles are aggregating over time, they may settle out of suspension and throw off the analysis or they may simply yield different results at different times and under different conditions. To help understanding about the sample stability, ELS is used to determine the zeta potential of particles in solution. Zeta potential works by applying a voltage to a sample of interest, measuring its rate of motion, and then correlating that to its zeta potential or effective charge in solution. This voltage, if applied too powerfully or for too long, can easily damage or degrade protein or nucleic acid samples. New developments in zeta potential technologies, such as the cmPALS technology from Anton Paar, help eliminate this issue by enabling much faster measurements and measurement at lower potentials. Additionally, new cuvette designs with an Omega-shaped sample loop also help limit protein loss when compared to the more traditional U-shaped cuvettes.
Betty Petrillo is the International Product Manager for the particle characterization product line at Anton Paar. She has been part of the development team for the Litesizer™ series since 2013 focusing also on the optimization and marketing activities of this product line.
She holds a PhD in polymer chemistry and drug delivery from Cardiff University (Uk) and gained a broad range of work experience in different fields before joining Anton Paar GmbH.
09:00 – Introduction and technical presentation
10:00 – Coffee break
10:20 – Instrument presentation
10:40 – Practical Session
11:30 – Discuss Topics
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